Title: Evaluation of monocot and eudicot divergence using the sugarcane transcriptome(1[w])

Author(s):  Vincentz M, Cara FAA, Okura VK, da Silva FR, Pedrosa GL, Hemerly AS, Capella AN, Marins M, Ferreira PC, Franca SC, Grivet L, Vettore AL, Kemper EL, Burnquist WL, Targon MLP, Siqueira WJ, Kuramae EE, Marino CL, Camargo LEA, Carrer H, Coutinho LL , Furlan LR, Lemos MVF, Nunes LR, Gomes SL, Santelli RV, Goldman MH, Bacci M, Giglioti EA, Thiemann OH, Silva FH, Van Sluys MA, Nobrega FG, Arruda P, Menck CFM

Source:  PLANT PHYSIOLOGY 134 (3): 951-959 MAR 2004

Document Type:  Article

Language:  English

Abstract:  Over 40,000 sugarcane (Saccharum officinarum) consensus sequences assembled from 237,954 expressed sequence tags were compared with the protein and DNA sequences from other angiosperms, including the genomes of Arabidopsis and rice (Oryza sativa). Approximately two-thirds of the sugarcane transcriptome have similar sequences in Arabidopsis. These sequences may represent a core set of proteins or protein domains that are conserved among monocots and eudicots and probably encode for essential angiosperm. functions. The remaining sequences represent putative monocot-specific genetic material, one-half of which were found only in sugarcane. These monocot-specific cDNAs represent either novelties or, in many cases, fast-evolving sequences that diverged substantially from their eudicot homologs. The wide comparative genome analysis presented here provides information on the evolutionary changes that underlie the divergence of monocots and eudicots. Our comparative analysis also led to the identification of several not yet annotated putative genes and possible gene loss events in Arabidopsis. KeyWords Plus:  SEQUENCE TAG COLLECTION; COMPARATIVE GENOMICS; GENE LOSS; FUNCTIONAL ANNOTATION; DRAFT SEQUENCE; TROPICAL CROP; ARABIDOPSIS; EVOLUTION; RICE; DNA

Addresses:  Menck CFM (reprint author), Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, 1374 Avenida Professor Lineu Prestes 1374, Sao Paulo, BR-05508900 Brazil
Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo, BR-05508900 Brazil
Univ Fed Paraiba, Inst Pesquisa & Desenvolvimento, Lab Genet Mol & Genomas, Sao Jose Dos Campos, SP BR-12244000 Brazil
Univ Sao Paulo, Inst Biociencias, Dept Bot, Sao Paulo, SP BR-05508900 Brazil
Univ Fed Sao Carlos, Dept Genet & Evolucao, Sao Carlos, SP BR-13565905 Brazil
Univ Sao Paulo, Inst Fis Sao Carlos, Sao Carlos, SP BR-13560970 Brazil
Univ Fed Sao Carlos, Ctr Ciencias Agrarias, Dept Biotecnol Vegetal, Araras, SP BR-13600970 Brazil
Univ Estadual Paulista, Inst Biociencias, Ctr Estudos Insetos Sociais, Rio Claro, SP BR-13506900 Brazil
Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Pret, Dept Biol, Ribeirao Preto, SP BR-14040901 Brazil
Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, BR-05508900 Brazil
Univ Mogi Cruzes, Nucleo Integrado Biotecnol, Mogi Cruzes, SP BR-08780911 Brazil
Univ Estadual Paulista, Fac Ciencias Agrarias & Vet Jaboticabal, Dept Biol Aplicada & Agropecuaria, Jaboticabal, SP BR-14884900 Brazil
Univ Estadual Paulista, Fac Ciencias Agrarias & Vet Jaboticabal, Dept Tecnol, Jaboticabal, SP BR-14884900 Brazil
Univ Sao Paulo, Escola Super Agr Luiz Queiroz, Piracicaba, SP BR-13418900 Brazil
Univ Estadual Paulista, Inst Biociencias, Dept Genet, Botucatu, SP BR-18618000 Brazil
Univ Estadual Paulista, Fac Ciencias Agronom, Dept Defesa Fitossantaria, Botucatu, SP BR-18603970 Brazil
Inst Agron Campinas, Ctr Genet Biol Mol & Fitoquim, Campinas, SP BR-13001970 Brazil
Inst Agronom Campinas, Ctr APTA Citros Sylvio Moreira, Cordeiropolis, SP BR-13490970 Brazil
Ctr Tecnol Copersucar, Piracicaba, SP BR-13400970 Brazil
Univ Ribeirao Preto, Dept Biotecnol Plantas Med, Ribeirao Preto, SP BR-14096380 Brazil
Univ Fed Rio de Janeiro, Ctr Ciencias Saude, Dept Bioquim, Rio De Janeiro, RJ Brazil
Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Campinas, SP BR-13083970 Brazil
Univ Estadual Campinas, Lab Bioinformat, Inst Comp, Campinas, SP BR-13083970 Brazil

E-mail Addresses: cfmmenck@usp.br

Publisher:  AMER SOC PLANT BIOLOGISTS, 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA

Subject Category:  PLANT SCIENCES

IDS Number:  805IQ

ISSN:  0032-0889