Title: Genetic characterization of Aberdeen Angus cattle using molecular markers

Author(s):  Vasconcellos LPDK, Tambasco-Talhari D, Pereira AP, Coutinho LL , Regitano LCD

Source:  GENETICS AND MOLECULAR BIOLOGY 26 (2): 133-137 JUN 2003

Document Type:  Article

Language:  English

Abstract:  Aberdeen Angus beef cattle from the Brazilian herd were studied genetically using restriction fragment length polymorphism (RFLP) of the K-casein - HinfI (CSAG - HinfI), beta-lactoglobulin - HaeIII (LGB - HaeIII) and growth hormone AluI (GH- AluI) genes, as well as four microsatellites (TEXAN 15, CSFM50, BM1224 and BM7160). The RFLP genotypes were determined using the polymerase chain reaction (PCR) followed by digestion with restriction endonucleases and electrophoresis in agarose gels. With the exception of the microsatellite BM7160, which was analyzed in an automatic sequencer, the PCR products were genotyped by silver staining. The allele and genotype frequencies, heterozygosities and gene diversity were estimated, The values for these parameters of variability were comparable to other cattle breeds. The genetic relationship of the Aberdeen Angus to other breeds (Caracu, Canchim, Charolais, Guzerath, Gyr, Nelore, Santa Gertrudis and Simmental) was investigated using Nei's genetic distance. Cluster analysis placed the Aberdeen Angus in an isolated group in the Bos taurus breeds branch. This fact is in agreement with the geographic origin of this breed.

Author Keywords:  Aberdeen Angus; bovine; DNA; characterization; RFLP; microsatellite

KeyWords Plus:  GROWTH-HORMONE GENOTYPES; BOVINE MICROSATELLITES; KAPPA-CASEIN; LINKAGE MAP; DNA; POLYMORPHISMS; CANCHIM; ALLELES; BULLS; LOCI

Addresses:  Regitano LCD (reprint author), Rodovia Washington Luiz Km 234,Caixa Postal 339, Sao Carlos, SP BR-13560970 Brazil
Univ Fed Sao Carlos, Programa Posgrad Genet & Evolucao, Sao Carlos, SP BR-13560 Brazil
USP, Escola Super Agr Luiz de Queiroz, Dept Anim Prod, Piracicaba, SP Brazil
Embrapa Pecuaria Sudeste, Lab Biotecnol Anim, Sao Carlos, SP Brazil

Publisher:  SOC BRASIL GENETICA, RUA CAP ADELMIO NORBET DA SILVA, 736, ALTO DA BOA VISTA, 14025-670 RIBEIRAO PRET, BRAZIL

Subject Category:  BIOCHEMISTRY & MOLECULAR BIOLOGY; GENETICS & HEREDITY

IDS Number:  702WW

ISSN:  1415-4757