Title: Expression of MyoD, myogenin, myostatin and Hsp70 transcripts in chicken embryos submitted to mild cold or heat

Author(s):  Gabriel JE, Alvares LE, Gobet MC, de Paz CCP, Packer IU, Macari A, Coutinho LL

Source:  JOURNAL OF THERMAL BIOLOGY 28 (4): 261-269 MAY 2003

Document Type:  Article

Language:  English

Abstract:  The expression of the MyoD, myogenin, myostatin and Hsp70 genes was estimated in chicken embryos submitted to mild cold (36 +/- 0.5degreesC) or heat (44 +/- 0.5degreesC) for 1 h. 2. Marked decreases in MyoD, myogenin and myostatin transcript levels were observed in embryos exposed to high temperature, contrasting to the higher expression of the Hsp70 mRNA detected in heat-stressed embryos. 3. The exposure of chicken embryos to low temperature significantly affected only the abundance of myogenin mRNA. 4. These findings suggest that myogenic proliferation and differentiation events are compromised by variations in environmental temperature during avian embryogenesis. (C) 2003 Elsevier Science Ltd. All rights reserved.

Author Keywords:  chicken embryos; Hsp70; MyoD; myogenin; myostatin

KeyWords Plus:  MESSENGER-RNA; SHOCK RESPONSE; NUCLEOTIDE-SEQUENCE; MUSCLE DEVELOPMENT; GENE-EXPRESSION; STRESS; PROTEINS; CELLS; DIFFERENTIATION; HSP90-ALPHA

Addresses:  Coutinho LL (reprint author), USP, ESALQ, Lab Biotecnol Anim, Dept Anim Prod, Av Padua Dias,11-CEP, Piracicaba, SP BR-13418900 Brazil
USP, ESALQ, Lab Biotecnol Anim, Dept Anim Prod, Piracicaba, SP BR-13418900 Brazil
Inst Anim Sci, IZ, Nova Odessa, BR-13460000 Brazil
UNESP, FCAVJ, Dept Anim Morphol & Physiol, Jaboticabal, BR-14870000 Brazil

Publisher:  PERGAMON-ELSEVIER SCIENCE LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND

Subject Category:  BIOLOGY; ZOOLOGY

IDS Number:  681LH

ISSN:  0306-4565