Title: Comparative analyses of the complete genome sequences of Pierce's disease and citrus variegated chlorosis strains of Xylella fastidiosa

Author(s):  Van Sluys MA, de Oliveira MC, Monteiro-Vitorello CB, Miyaki CY, Furlan LR, Camargo LEA, da Silva ACR, Moon DH, Takita MA, Lemos EGM, Machado MA, Ferro MIT, da Silva FR, Goldman MHS, Goldman GH, Lemos MVF, El-Dorry H, Tsai SM, Carrer H, Carraro DM, de Oliveira RC, Nunes LR, Siqueira WJ, Coutinho LL , Kimura ET, Ferro ES, Harakava R, Kuramae EE, Marino CL, Giglioti E, Abreu IL, Alves LMC, do Amaral AM, Baia GS, Blanco SR, Brito MS, Cannavan FS, Celestino AV, da Cunha AF, Fenille RC, Ferro JA, Formighieri EF, Kishi LT, Leoni SG, Oliveira AR, Rosa VE, Sassaki FT, Sena JAD, de Souza AA, Truffi D, Tsukumo F, Yanai GM, Zaros LG, Civerolo EL, Simpson AJG, Almeida NF, Setubal JC, Kitajima JP

Source:  JOURNAL OF BACTERIOLOGY 185 (3): 1018-1026 FEB 2003

Document Type: Article

Language:  English

Abstract:  Xylella fastidiosa is a xylem-dwelling, insect-transmitted, gamma-proteobacterium that causes diseases in many plants, including grapevine, citrus, periwinkle, almond, oleander, and coffee. X. fastidiosa has an unusually broad host range, has an extensive geographical distribution throughout the American continent, and induces diverse disease phenotypes. Previous molecular analyses indicated three distinct groups of X.fastidiosa isolates that were expected to be genetically divergent. Here we report the genome sequence of X. fastidiosa (Temecula strain), isolated from a naturally infected grapevine with Pierce's disease (PD) in a wine-grape-growing region of California. Comparative analyses with a previously sequenced X.fastidiosa strain responsible for citrus variegated chlorosis (CVC) revealed that 98% of the PD X.fastidiosa Temecula genes are shared with the CVC X. fastidiosa strain 9a5c genes. Furthermore, the average amino acid identity of the open reading frames in the strains is 95.7%. Genomic differences are limited to phage-associated chromosomal rearrangements and deletions that also account for the strain-specific genes present in each genome. Genomic islands, one in each genome, were identified, and their presence in other X.fastidiosa strains was analyzed. We conclude that these two organisms have identical metabolic functions and are likely to use a common set of genes in plant colonization and pathogenesis, permitting convergence of functional genomic strategies.

KeyWords Plus:  GENETIC DIVERSITY; 16S RDNA; PATHOGEN; IDENTIFICATION; ISLAND; FAMILY

Addresses:  Van Sluys MA (reprint author), Univ Sao Paulo, Inst Biociencias, Dept Bot, R do Matao 277, Sao Paulo, BR-05508900 Brazil
Univ Sao Paulo, Inst Biociencias, Dept Bot, Sao Paulo, BR-05508900 Brazil
Univ Sao Paulo, Inst Quim, Sao Paulo, BR-05508900 Brazil
Univ Sao Paulo, Inst Ciencias Biomed, Sao Paulo, BR-05508900 Brazil
Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Piracicaba, BR-13418900 Brazil
Univ Sao Paulo, Ctr Energia Nucl Agr, Piracicaba, BR-13400970 Brazil
Inst Agron Campinas, Ctr Citricultura Sylio Moreira, Cordeiropolis, BR-13490970 Brazil
Univ Estadual Paulista, Fac Ciencias Agr & Vet, Jaboticabal, BR-14884900 Brazil
Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Campinas, SP BR-13083970 Brazil
Univ Estadual Campinas, Inst Biol, Campinas, SP BR-13083970 Brazil
Univ Estadual Campinas, Inst Comp, Campinas, SP BR-13083970 Brazil
Univ Sao Paulo, Fac Ciencias Farmaceut, Ribeirao Preto, BR-14040901 Brazil
Univ Sao Paulo, Fac Filosofia Ciencias & Letras, Ribeirao Preto, BR-14040901 Brazil
Inst Ludwig Pesquisa Canc, Sao Paulo, BR-01509010 Brazil
Univ Mogi das Cruzes, Nucleo Integrado Biotecnol, Mogi das Cruzes, BR-08780911 Brazil
Inst Agron Campinas, Campinas, SP BR-13001970 Brazil
Inst Biol, Sao Paulo, BR-04014002 Brazil
Univ Estadual Paulista, Fac Med Vet & Zootecnia, Botucatu, SP BR-18603970 Brazil
Univ Estadual Paulista, Fac Ciencias Agron, Botucatu, SP BR-18603970 Brazil
Univ Estadual Paulista, Inst Biociencias, Botucatu, SP BR-18618000 Brazil
Univ Fed Sao Carlos, Sao Paulo, BR-13600970 Brazil
Univ Fed Mato Grosso do Sul, Dept Comp & Estatist, Campo Grande, MS BR-79070900 Brazil
USDA ARS, Parlier, CA 93648 USA

Publisher:  AMER SOC MICROBIOLOGY, 1752 N ST NW, WASHINGTON, DC 20036-2904 USA Subject Category:  MICROBIOLOGY

IDS Number:  642YG

ISSN:  0021-9193