Title: Distribution of bovine lymphocyte antigen (BOLA-DRB3) alleles in Brazilian dairy Gir cattle (Bos indicus)

Author(s):  da Mota AF, Gabriel JE, Martinez ML, Coutinho LL

Source:  EUROPEAN JOURNAL OF IMMUNOGENETICS 29 (3): 223-227 JUN 2002

Document Type:  Article

Language:  English

Abstract:  Brazilian dairy Gir (Bus indices) cattle are a tropical, well-adapted breed, for which no information on the major histocompatibility complex (MHC) is presently available. The second exon of the bovine lymphocyte antigen (BOLA-DRB3) was amplified by polymerase chain reaction (PCR) of DNA samples from 28 Brazilian dairy Gir cows. Two experimental genotyping approaches were used: direct sequencing of PCR gene products (PCR-DS) and sequencing of cloned PCR fragments (S-CLO). Results demonstrate the viability of both typing approaches. PCR-DS allowed typing of 39% of the animals while the remainder were genotyped by S-CLO. Seventeen BOLA-DRB3 alleles were assigned, including some that were only recently described for zebu cattle. Allelic frequencies ranged from 0.02 to 0.18. The most frequent alleles were *3501 (frequency = 0.18), *2201 (0.14) and *2101 (0.11).

KeyWords Plus:  GENETIC ASSOCIATION; HOLSTEIN CATTLE; DRB3.2 ALLELES; PCR-RFLP; MILK; POLYMORPHISM; FREQUENCIES; COWS

Addresses:  Coutinho LL (reprint author), USP, ESALQ, Dept Prod Anim, Lab Biotecnol Anim, Av Padua Dias 11, Piracicaba, SP BR-13418900 Brazil
USP, ESALQ, Dept Prod Anim, Lab Biotecnol Anim, Piracicaba, SP BR-13418900 Brazil
Brazilian Org Agr Res, Natl Dairy Cattle Res Ctr, Embrapa Gado de Leite, Juiz de Fora, MG BR-36038330 Brazil

Publisher:  BLACKWELL PUBLISHING LTD, 9600 GARSINGTON RD, OXFORD OX4 2DG, OXON, ENGLAND

Subject Category:  GENETICS & HEREDITY; IMMUNOLOGY

IDS Number:  566QE

ISSN:  0960-7420