Title: Comparison of the genomes of two Xanthomonas pathogens with differing host specificities

Author(s):  da Silva ACR, Ferro JA, Reinach FC, Farah CS, Furlan LR, Quaggio RB, Monteiro-Vitorello CB, Van Sluys MA, Almeida NF, Alves LMC, do Amaral AM, Bertolini MC, Camargo LEA, Camarotte G, Cannavan F, Cardozo J, Chambergo F, Clapina LP, Cicarelli RMB, Coutinho LL , Cursino-Santos JR, El-Dorry H, Faria JB, Ferreira AJS, Ferreira RCC, Ferro MIT, Formighieri EF, Franco MC, Greggio CC, Gruber A, Katsuyama AM, Kishi LT, Leite RP, Lemos EGM, Lemos MVF, Locali EC, Machado MA, Madeira AMBN, Martinez-Rossi NM, Martins EC, Meidanis J, Menck CFM, Miyaki CY, Moon DH, Moreira LM, Novo MTM, Okura VK, Oliveira MC, Oliveira VR, Pereira HA, Rossi A, Sena JAD, Silva C, de Souza RF, Spinola LAF, Takita MA, Tamura RE, Teixeira EC, Tezza RID, dos Santos MT, Truffi D, Tsai SM, White FF, Setubal JC, Kitajima JP

Source:  NATURE 417 (6887): 459-463 MAY 23 2002

Document Type:  Article

Language:  English

Abstract:  The genus Xanthomonas is a diverse and economically important group of bacterial phytopathogens, belonging to the gamma-subdivision of the Proteobacteria. Xanthomonas axonopodis pv. citri (Xac) causes citrus canker, which affects most commercial citrus cultivars, resulting in significant losses worldwide. Symptoms include canker lesions, leading to abscission of fruit and leaves and general tree decline(1). Xanthomonas campestris pv. campestris (Xcc) causes black rot, which affects crucifers such as Brassica and Arabidopsis. Symptoms include marginal leaf chlorosis and darkening of vascular tissue, accompanied by extensive wilting and necrosis(2). Xanthomonas campestris pv. campestris is grown commercially to produce the exopolysaccharide xanthan gum, which is used as a viscosifying and stabilizing agent in many industries(3). Here we report and compare the complete genome sequences of Xac and Xcc. Their distinct disease phenotypes and host ranges belie a high degree of similarity at the genomic level. More than 80% of genes are shared, and gene order is conserved along most of their respective chromosomes. We identified several groups of strain-specific genes, and on the basis of these groups we propose mechanisms that may explain the differing host specificities and pathogenic processes.

KeyWords Plus:  CAMPESTRIS PV VESICATORIA; GENE-CLUSTER; LIPOPOLYSACCHARIDE BIOSYNTHESIS; PATHOVAR CAMPESTRIS; SECRETION SYSTEMS; EXPRESSION; SEQUENCE; PROTEINS; HRP; PERSPECTIVE

Addresses:  da Silva ACR (reprint author), Univ Sao Paulo, Inst Quim, Dept Bioquim, Av Prof Lineu Prestes 748, Sao Paulo, BR-05508900 Brazil
Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, BR-05508900 Brazil
Univ Sao Paulo, Inst Biociencias, Dept Bot, Sao Paulo, BR-05508900 Brazil
Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo, BR-05508900 Brazil
Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Patol, Sao Paulo, BR-05508900 Brazil
Univ Sao Paulo, Inst Biociencias, Dept Biol, Sao Paulo, BR-05508900 Brazil
Univ Estadual Paulista, Fac Ciencias Agrarias & Vet, Dept Tecnol, Jaboticabal, SP BR-14884900 Brazil
Univ Estadual Paulista, Fac Ciencias Agrarias & Vet, Dept Biol Aplicada Agropecuaria, Jaboticabal, SP BR-14884900 Brazil
Univ Estadual Paulista, Fac Med Vet & Zootecnia, Dept Melhoramento & Nutr Anim, Botucatu, SP BR-18600000 Brazil
Univ Sao Paulo, Escola Super Agr Luiz Queiroz, Piracicaba, SP Brazil
Univ Fed Mato Grosso Sul, Dept Computacao & Estatist, Campo Grande, MS BR-79070900 Brazil
IAC, Ctr Citricultura Sylvio Moreira, Cordeiropolis, SP BR-13490970 Brazil
Univ Estadual Paulista, Inst Quim, Dept Bioquim & Tecnol Quim, Araraquara, SP BR-14801970 Brazil
Ctr Energia Nucl Agr, Piracicaba, SP BR-13400970 Brazil
Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Ciencias Biol, Araraquara, SP BR-14801902 Brazil
Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP BR-14049900 Brazil
Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, Ribeirao Preto, SP BR-14049900 Brazil
IAPAR, Inst Tecnol Parana, Londrina, PR BR-86001970 Brazil
Univ Estadual Campinas, Inst Computacao, Ctr Biol Mol & Engn Genet, Campinas, SP BR-13084971 Brazil Publisher:  NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND

Subject Category:  MULTIDISCIPLINARY SCIENCES

IDS Number:  554HZ

ISSN:  0028-0836