Title: PCR screening and allele frequency estimation of bovine leukocyte adhesion deficiency in Holstein and Gir cattle in Brazil

Author(s):  Ribeiro LA, Baron EE, Martinez ML, Coutinho LL

Source:  GENETICS AND MOLECULAR BIOLOGY 23 (4): 831-834 DEC 2000
Document Type:  Article

Language:  English

Abstract:  Bovine leukocyte adhesion deficiency (BLAD) is a disease known to affect the Holstein cattle breed throughout the world. Eighty-eight Holstein dairy cows and 88 Gir dairy bulls were genotyped by PCR for the CD 18 BLAD alelle. The frequency of the BLAD mutant allele and the BLAD-carrier prevalence in Brazilian Holstein cows were 2.8 and 5.7%, respectively. No mutant allele was found in any of the 88 Gir animals. We conclude that the CD 18 gene mutation is prevalent in Brazilian Holstein cattle and absent or present at a very low frequency in Gir cattle.

KeyWords Plus:  FRIESIAN CATTLE; BLAD; IDENTIFICATION; PREVALENCE; MAC-1; GENE

Addresses:  Coutinho LL (reprint author), USP, ESALQ, Dept Anim Prod, Caixa Postal 9, Piracicaba, SP BR-13418900 Brazil
USP, ESALQ, Dept Anim Prod, Piracicaba, SP BR-13418900 Brazil
EMBRAPA, Ctr Natl Pesquisa Gado Leite, Juiz De Fora, MG BR-36038330 Brazil Publisher:  SOC BRASIL GENETICA, RUA CAP ADELMIO NORBET DA SILVA, 736, ALTO DA BOA VISTA, 14025-670 RIBEIRAO PRET, BRAZIL

Subject Category:  BIOCHEMISTRY & MOLECULAR BIOLOGY; GENETICS & HEREDITY

IDS Number:  468QX

ISSN:  1415-4757