Title: The genome sequence of the plant pathogen Xylella fastidiosa

Author(s):  Simpson AJG, Reinach FC, Arruda P, Abreu FA, Acencio M, Alvarenga R, Alves LMC, Araya JE, Baia GS, Baptista CS, Barros MH, Bonaccorsi ED, Bordin S, Bove JM, Briones MRS, Bueno MRP, Camargo AA, Camargo LEA, Carraro DM, Carrer H, Colauto NB, Colombo C, Costa FF, Costa MCR, Costa-Neto CM, Coutinho LL , Cristofani M, Dias-Neto E, Docena C, El-Dorry H, Facincani AP, Ferreira AJS, Ferreira VCA, Ferro JA, Fraga JS, Franca SC, Franco MC, Frohme M, Furlan LR, Garnier M, Goldman GH, Goldman MHS, Gomes SL, Gruber A, Ho PL, Hoheisel JD, Junqueira ML, Kemper EL, Kitajima JP, Krieger JE, Kuramae EE, Laigret F, Lambais MR, Leite LCC, Lemos EGM, Lemos MVF, Lopes SA, Lopes CR, Machado JA, Machado MA, Madeira AMBN, Madeira HMF, Marino CL, Marques MV, Martins EAL, Martins EMF, Matsukuma AY, Menck CFM, Miracca EC, Miyaki CY, Monteiro-Vitorello CB, Moon DH, Nagai MA, Nascimento ALTO, Netto LES, Nhani A, Nobrega FG, Nunes LR, Oliveira MA, de Oliveira MC, de Oliveira RC, Palmieri DA, Paris A, Peixoto BR, Pereira GAG, Pereira HA, Pesquero JB, Quaggio RB, Roberto PG, Rodrigues V, Rosa AJD, de Rosa VE, de Sa RG, Santelli RV, Sawasaki HE, da Silva ACR, da Silva AM, da Silva FR, Silva WA, da Silveira JF, Silvestri MLZ, Siqueira WJ, de Souza AA, de Souza AP, Terenzi MF, Truffi D, Tsai SM, Tsuhako MH, Vallada H, Van Sluys MA, Verjovski-Almeida S, Vettore AL, Zago MA, Zatz M, Meidanis J, Setubal JC

Group Author(s):  Xylella fastidiosa Consortium Org Source:  NATURE 406 (6792): 151-157 JUL 13 2000

Document Type:  Article

Language:  English

Abstract:  Xylella fastidiosa is a fastidious, xylem-limited bacterium that causes a range of economically important plant diseases. Here we report the complete genome sequence of X. fastidiosa clone 9a5c, which causes citrus variegated chlorosis-a serious disease of orange trees. The genome comprises a 52.7% GC-rich 2,679,305-base-pair (bp) circular chromosome and two plasmids of 51,158 bp and 1,285 bp. We can assign putative functions to 47% of the 2,904 predicted coding regions. Efficient metabolic functions are predicted, with sugars as the principal energy and carbon source, supporting existence in the nutrient-poor xylem sap. The mechanisms associated with pathogenicity and virulence involve toxins, antibiotics and ion sequestration systems, as well as bacterium-bacterium and bacterium-host interactions mediated by a range of proteins. Orthologues of some of these proteins have only been identified in animal and human pathogens; their presence in X. fastidiosa indicates that the molecular basis for bacterial pathogenicity is both conserved and independent of host. At least 83 genes are bacteriophage-derived and include virulence-associated genes from other bacteria, providing direct evidence of phage-mediated horizontal gene transfer.

KeyWords Plus:  CITRUS VARIEGATED CHLOROSIS; VIRULENCE FACTORS; SWEET ORANGE; HAEMOPHILUS-INFLUENZAE; DICHELOBACTER-NODOSUS; LIMITED BACTERIUM; ESCHERICHIA-COLI; RTX TOXINS; IDENTIFICATION; SECRETION

Addresses:  Setubal JC (reprint author), Univ Estadual Campinas, Inst Computacao, Caixa Postal 6176, Campinas, SP BR-13083970 Brazil
Univ Estadual Campinas, Inst Computacao, Campinas, SP BR-13083970 Brazil
Inst Ludwig Pesquisa Canc, Sao Paulo, BR-01509010 Brazil
Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, BR-05508900 Brazil
Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Campinas, SP BR-13083970 Brazil
Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Patol, Mol Biol Lab, Sao Paulo, BR-05508000 Brazil
Univ Sao Paulo, Fac Med, Dept Radiol, Disciplina Oncol, Sao Paulo, BR-01296903 Brazil
Univ Estadual Paulista, Fac Ciencias Agr & Vet Jaboticabal, Dept Tecnol, Jaboticabal, SP BR-14884900 Brazil
Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, BR-04023062 Brazil
Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo, BR-05508900 Brazil
Univ Estadual Campinas, Fac Ciencias Med, Hemoctr, Campinas, SP BR-13083970 Brazil
INRA, Vilenave Dornon, F-33883 France
Univ Bordeaux 2, Biol Cellulaire & Mol Lab, Vilenave Dornon, F-33883 France
Univ Sao Paulo, Inst Biociencias, Dept Biol, Sao Paulo, BR-05508900 Brazil
Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Piracicaba, SP BR-13418900 Brazil
Univ Estadual Paulista, Inst Biociencias, Dept Genet, Botucatu, SP BR-18618000 Brazil
Inst Agron Campinas, Ctr Genet Biol Mol & Fitoquim, Campinas, SP BR-13001970 Brazil
Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Clin Med, Ribeirao Preto, SP BR-14049900 Brazil
Univ Fed Sao Paulo, Escola Paulista Med, Dept Biofis, Sao Paulo, BR-04023062 Brazil
Inst Agron Campinas, Ctr Citricultura Sylvio Moreira, Cordeiropolis, SP BR-13490970 Brazil
Inst Biol, Lab Bioquim Fitopatol, Sao Paulo, BR-04014002 Brazil
Inst Biol, Lab Imunol, Sao Paulo, BR-04014002 Brazil
Univ Ribeirao Preto, Dept Biotecnol Plantas Med, Ribeirao Preto, SP BR-14096380 Brazil
Univ Sao Paulo, Ctr Energia Nucl Agr, Piracicaba, SP BR-13400970 Brazil
Deutsch Krebsforschungszentrum, Heidelberg, D-69120 Germany
Univ Estadual Paulista, Fac Med Vet & Zootecnia, Dept Melhoramento & Nutr, Botucatu, SP BR-18600000 Brazil
Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, Ribeirao Preto, SP BR-14040903 Brazil
Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Pret, Dept Biol, Ribeirao Preto, SP BR-14040901 Brazil
Inst Butantan, Ctr Biotecnol, Sao Paulo, BR-05503900 Brazil
Univ Sao Paulo, Fac Med, Inst Coracao, Lab Genet & Cardiol Mol LIM 13, Sao Paulo, BR-05403000 Brazil
Univ Estadual Paulista, Fac Ciencias Agron, Dept Prod Vegetal, Botucatu, SP BR-18603970 Brazil
Univ Estadual Paulista, Fac Ciencias Agr & Vet Jaboticabal, Dept Biol Aplicada Agropecuaria, Jaboticabal, SP BR-14884900 Brazil
Hosp Canc AC Camargo, Sao Paulo, BR-01509010 Brazil
Univ Mogi das Cruzes, Nucleo Integrado Biotecnol, Mogi Das Cruzes, SP BR-08780911 Brazil
Univ Estadual Campinas, Inst Biol, Dept Genet & Evolucao, Campinas, SP BR-13083970 Brazil
Univ Sao Paulo, Inst Biociencias, Dept Bot, Sao Paulo, BR-05508900 Brazil
Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Parasitol Microbiol & Imunol, Ribeirao Preto, SP BR-14049900 Brazil
Univ Sao Paulo, Fac Med, Inst Psiquiatria, Dept Psiquiatria, Sao Paulo, BR-05403010 Brazil
Univ Vale Paraiba, Sao Jose dos Campos, Brazil Publisher:  MACMILLAN PUBLISHERS LTD, PORTERS SOUTH, 4 CRINAN ST, LONDON N1 9XW, ENGLAND

Subject Category:  MULTIDISCIPLINARY SCIENCES

IDS Number:  335BA

ISSN:  0028-0836