Title: In situ detection of transcripts of the myogenic factor MyoD in whole chicken embryos

Author(s):  Gabriel JE, Alves HJ, Alvares LE, Schmidt GS, Coutinho LL

Source:  GENETICS AND MOLECULAR BIOLOGY 23 (1): 145-148 MAR 2000

Document Type:  Article

Language:  English

Abstract:  In situ hybridization has provided insights into the molecular basis of skeletal myogenesis during embryonic development. In situ detection of different muscle-specific regulatory factors in whole embryos has been described. Spatial and temporal expression patterns of these factors differed among species, The expression pattern of MyoD in whole chicken embryos was studied via in situ hybridization using a probe obtained by the reverse transcription - polymerase chain reaction (RT-PCR) technique. In newly formed somites (embryos of stage 12), MyoD mRNA transcripts were detected along the anterior to posterior axis of somites immediately adjacent to the neural tube, whereas in mature somites (embryos of stage 24), MyoD transcripts were detected throughout the entire somite. These results indicate that MyoD expression is important for initiating and maintaining the avian myogenic system.

KeyWords Plus:  MYOSIN HEAVY-CHAIN; QUAIL EMBRYOS; EXPRESSION; SOMITE; GENES; ACTIVATION; QMF1

Addresses:  Coutinho LL (reprint author), USP, ESALQ, Dept Zootecnia, Av Padua Dias 11, Piracicaba, SP BR-13418900 Brazil
USP, ESALQ, Dept Zootecnia, Piracicaba, SP BR-13418900 Brazil
EMBRAPA, Concordia, SC Brazil Publisher:  SOC BRASIL GENETICA, RUA CAP ADELMIO NORBET DA SILVA, 736, ALTO DA BOA VISTA, 14025-670 RIBEIRAO PRET, BRAZIL

Subject Category:  BIOCHEMISTRY & MOLECULAR BIOLOGY; GENETICS & HEREDITY

IDS Number:  313MB

ISSN:  1415-4757