Title: Identification of proliferating cells in chicken embryos using 5-bromo-2 '-deoxyuridine immunohistochemical detection

Author(s):  Lopes ML, Schmidt GS, Coutinho LL

Source:  GENETICS AND MOLECULAR BIOLOGY 23 (1): 149-153 MAR 2000

Document Type:  Article

Language:  English

Abstract:  Chicken embryos were incubated with BrdU, embedded in plastic resin, sectioned and screened immunohistochemically to identify proliferating cells in the neural tube and somites. Fixation in 4% paraformaldehyde for 1 h was essential for detecting specific colorimetric signals of BrdU incorporation into cells during the S phase of the cell cycle. Transverse sections of the neural tube showed that the nuclei of proliferating cells (BrdU positive) had a uniform and centralized distribution, whereas unstained nuclei were found only along the extremities of the neural tube, Transverse sections of differentiated somites showed proliferating cells in the scleratome and dermatome. However, no incorporation of BrdU was observed in myotomic cells, which give rise to axial skeletal muscle, In spite of their proximity, the dermatome and myotome showed marked differences in cell proliferation. The excellent preservation of morphological characteristics in the embryonic tissues facilitated identification of variations in BrdU incorporation.

KeyWords Plus:  DNA

Addresses:  Coutinho LL (reprint author), USP, ESALQ, Dept Zootecnia, Av Padua Dias 11, Piracicaba, SP BR-13418900 Brazil
USP, ESALQ, Dept Zootecnia, Piracicaba, SP BR-13418900 Brazil
EMBRAPA, Concordia, SC Brazil Publisher:  SOC BRASIL GENETICA, RUA CAP ADELMIO NORBET DA SILVA, 736, ALTO DA BOA VISTA, 14025-670 RIBEIRAO PRET, BRAZIL

Subject Category:  BIOCHEMISTRY & MOLECULAR BIOLOGY; GENETICS & HEREDITY

IDS Number:  313MB

ISSN:  1415-4757