Title: In situ DNA transfer to chicken embryos by biolistics

Author(s):  Ribeiro LA, Azevedo JL, Aragao FJL, Rech EL, Schmidt GS, Coutinho LL

Source:  GENETICS AND MOLECULAR BIOLOGY 22 (4): 525-529 DEC 1999

Document Type:  Article

Language:  English

Abstract:  Fertilized chicken eggs were bombarded with a biolistic device. Transient expression of the lacZ gene under the control of a human cytomegalovirus (CMV) promoter was assessed after in situ gene transfer using this approach. The influence of different pressures, Vacuum levels and particles was tested. Survival rate improved as particle velocity decreased, but resulted in lower levels of expression. The best survival and expression were obtained with gold particles, a helium gas pressure of 600 psi and a Vacuum of 600 mmHg. Under these conditions, all bombarded embryos showed P-galactosidase activity, indicating that this was an effective method for transformation of chicken embryos. KeyWords Plus:  PARTICLE BOMBARDMENT; FOREIGN GENES; CELLS; EXPRESSION; TISSUES; MICROPROJECTILES; OVO

Addresses:  Coutinho LL (reprint author), USP, ESALQ, Escola Super Agr Luiz de Queiroz, Av Padua Dias 11,Caixa Postal 9, Piracicaba, SP BR-13418900 Brazil
USP, ESALQ, Escola Super Agr Luiz de Queiroz, Piracicaba, SP BR-13418900 Brazil
USP, ESALQ, Dept Genet, Piracicaba, SP BR-13418900 Brazil
EMBRAPA, Cenargen, SAIN Parque Rural, Brasilia, DF Brazil
Embrapa Suinos & Aves, Concordia, SC Brazil Publisher:  SOC BRASIL GENETICA, RUA CAP ADELMIO NORBET DA SILVA, 736, ALTO DA BOA VISTA, 14025-670 RIBEIRAO PRET, BRAZIL

Subject Category:  BIOCHEMISTRY & MOLECULAR BIOLOGY; GENETICS & HEREDITY

IDS Number:  268ZA

ISSN:  1415-4757