Title: Candidate gene analysis of GH1 for effects on growth and carcass composition of cattle

Author(s):  Taylor JF, Coutinho LL , Herring KL, Gallagher DS, Brenneman RA, Burney N, Sanders JO, Turner RV, Smith SB, Miller RK, Savell JW, Davis SK

Source:  ANIMAL GENETICS 29 (3): 194-201 JUN 1998

Document Type:  Article

Language:  English

Abstract:  We present an approach to evaluate the support for candidate genes as quantitative trait loci (QTLs) within the context of genome-wide map-based cloning strategies. To establish candidacy, a bacterial artificial chromosome (BAC) clone containing a putative candidate gene is physically assigned to an anchored linkage map to localise the gene relative to an identified QTL effect. Microsatellite loci derived from BAC clones containing an established candidate gene are integrated into the linkage map facilitating the evaluation by interval analysis of the statistical support for QTL identity. Permutation analysis is employed to determine experiment-wise statistical support. The approach is illustrated for the growth hormone 1 (GH1) gene and growth and carcass phenotypes in cattle. Polymerase chain reaction (PCR) primers which amplify a 441 bp fragment of GH1 were used to systematically screen a bovine BAC library comprising 60 000 clones and with a 95% probability of containing a single copy sequence. The presence of GH1 in BAC110R2C3 was confirmed by sequence analysis of the PCR product from this clone and by the physical assignment of BAC110R2C3 to bovine chromosome 19 (BTA19) band 22 by fluorescence in situ hybridisation (FISH). Microsatellite KHGH1 was isolated from BAC110R2C3 and scored in 529 reciprocal backcross and F-2 fullsib progeny from 41 resource families derived from Angus (Bos taurus) and Brahman (Bos indicus). The microsatellite KHGH1 was incorporated into a framework genetic map of BTA19 comprising 12 microsatellite loci, the erythrocyte antigen T and a GH1-TaqI restriction fragment length polymorphism (RFLP). Interval analysis localised effects of taurus vs, indicus alleles on subcutaneous fat and the percentage of ether extractable fat from the longissimus dorsi muscle to the region of BTA19 harbouring GH1. Author

Keywords:  bovine; BTA19; genetic linkage; GH1; interval analysis; permutation test

KeyWords Plus:  FRAGMENT-LENGTH-POLYMORPHISMS; QUANTITATIVE TRAIT LOCI; ASSOCIATIONS; MAP; MARKERS; LINKAGE; HORMONE; GENOME

Addresses:  Taylor JF (reprint author), Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA
Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA
Univ Sao Paulo, Esalq, Dept Zootecn, Sao Paulo, BR-13418900 Brazil Publisher:  BLACKWELL SCIENCE LTD, P O BOX 88, OSNEY MEAD, OXFORD OX2 0NE, OXON, ENGLAND Subject Category:  AGRICULTURE, DAIRY & ANIMAL SCIENCE; GENETICS & HEREDITY

IDS Number:  108CR

ISSN:  0268-9146