17 - JOURNAL OF EXPERIMENTAL ZOOLOGY 294 (4): 346-351 DEC 15 2002
Zucoloto RB, Verdade LM, Coutinho LL
Title: Microsatellite DNA library for Caiman latirostrisAuthor(s): Zucoloto RB, Verdade LM, Coutinho LLSource: JOURNAL OF EXPERIMENTAL ZOOLOGY 294 (4): 346-351 DEC 15 2002Document Type: ArticleLanguage: EnglishAbstract: New genetic markers were characterized for the broad-snouted caiman (Caiman latirostris) by constructing libraries enriched for microsatellite DNA. Construction and characterization of these libraries are described in the present study. One microsatellite marker was developed from a (ACC-TGG)(n) enriched microsatellite DNA library, and 12 microsatellite markers were developed from a (AC-TG)(n) enriched microsatellite DNA library. These markers were tested in wild-caught animals, and these tests resulted in ten new polymorphic microsatellites for C. latirostris. (C) 2002 Wiley-Liss, Inc. KeyWords Plus: LOCIAddresses: Coutinho LL (reprint author), ESALQ USP, LPA, Biotechnol Lab, POB 9 Piracicaba, Sao Paulo, BR-13418900 BrazilESALQ USP, LPA, Biotechnol Lab, Sao Paulo, BR-13418900 BrazilCENA USP, Piracicaba, SP BR-13400 BrazilESALQ USP, LPA, Lab Ecol Anim, Piracicaba, SP BR-13418 BrazilPublisher: WILEY-LISS, DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USASubject Category: ZOOLOGYIDS Number: 621JLISSN: 0022-104X